Beads were sequentially hybridised (1 h, 50°C) with Pre-Amplifier probe, the Amplifier probe, the label probe and SAPE with washes in between followed by a final 30 min incubation at 37°C.
To decrease reagent cost and improve reagent durability, we set the hard constraint that RNA probes and amplifiers be replaced by new DNA probes and amplifiers engineered to be suitable for use in situ.
In situ hybridization protocols, nonspecific hairpin binding studies, in silico and in vitro analysis of DNA HCR amplifiers, characterization of background and signal in situ, probe sequences, and HCR amplifier sequences.
The device consists of fibre optic transmitter, fibre optic probe, mini-shaker, power amplifier, dynamic signal analyser and photodiode detector.
The diffusion NMR experiments were carried out with a Bruker Avance™ 600 MHz system equipped with a 5-mm Diff30 water-cooled diffusion probe and a gradient amplifier capable of achieving gradient strength up to 1,200 G/cm.
The micro-manipulator motor controls the laser, the ambient light on the PSD, the PSD board and its connections, the amplifiers, the probe, the dSPACE panel and control board which is used to get the data from the PSD board and finally any movements in the area that may cause the PSD or the laser to shake.
Porcine ACTB and 18S rRNA amplifier set probes were used as endogenous control for normalisation.
The samples were then incubated successively in three working solutions: pre-amplifier and amplifier, biotinylated probes and SAPE.
Antennae from blood-fed female mosquitoes were placed in EAG probes of an AM-01 amplifier (Syntech) and held in place with Spectra 360 electrode gel (Parker Laboratories, Orange, NJ).
The electrode was connected to a high impedance probe, which was connected to an amplifier.
