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DNA probe 1 (P1) was assembled on Au nanoparticles (AuNPs) modified electrode.
The evaluated pKa value of the probe 1 is 4.23.
The single probe 1 exhibited strong green fluorescence emission.
Probe 1 is pH-independent at the physiological pH range.
We utilized p16 probe 1 to detect methylated DNA.
Scheme 1 Strategy of probe 1 for sulfatase activity detection.
Streptavidin AuNPs coated with biotinylated DNA capture probe 1 (AuNPs probe 1) were firstly assembled onto an gold electrode through 1,3-propanedithiol.
A fluorescent probe 1 for Hg2+ based on a rhodamine coumarin conjugate was designed and synthesized.
The probe 1 exhibited distinct red fluorescence and good stability under physiological pH.
Probe 1 could quantificationally detect Hg2+ by the absorption and fluorescence spectroscopy methods with excellent sensitivity.
Moreover, probe 1 could detect H2S quantitatively with a detection limit as low as 0.23 μM.
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