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Although Peatman and Liu also described the presence of large MIP and MCP CC chemokine groups in their tree [ 36], such groups are not found in our tree most probably due to the usage of different alignment and tree construction methods.
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This is probably due to the use of polyunsaturated lipids.
Probably due to the differences in codon-usage or caused by the high proliferation as well as expression rates, the specific activities of the not codon-optimized d-Hyd were comparatively low for all cultivation conditions.
Contrary to our findings, they did not find differences in prognosis and mutation status in this subgroup, probably due to the low representation of this usage.
However because of the fragility of agarose, the activity of immobilized enzyme decreased sharply after multiple usages probably due to the structure crash of agarose.
Indeed the other pair of parental (EDM49c) and evolved (EDM101c) strains displayed practically no divergence in gene content or codon usage bias, probably due to the fact that they were isolated only 7 days apart.
This is probably due to the incomplete NlaIII digestion during library preparation and the usage of alternative polyadenylation and/or splicing sites [ 28].
We found that approximately 80% of the tags mapped to a CTAG site (data not shown), this is probably due to the incomplete NlaIII digestion during library preparation and the usage of alternative polyadenylation and/or splicing sites [ 52].
The higher usage of sulfonylurea is probably due to the need to rapidly control the glucose levels and the preference for glimepiride could be due to its lower propensity to cause hypoglycaemia.
Although a LAMA and LABA can be administered together using separate inhalers (i.e. as a 'free combination'), this usage is not common, probably due to the inconvenience of handling separate devices [ 4]; delivery of medication with a single device is always more convenient for the patient.
The intense usage of the paper version was probably due to the recruitment procedure, which included sending off the paper-based questionnaire.
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