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The output levels of probably damaging and possibly damaging were classified as functionally significant (≤0.5) and the benign level being classified as tolerated (≥0.51) [ 38].
Predictions are characterized as either benign, possibly damaging or probably damaging on the output.
The classification of "possibly damaging" and "probably damaging" prompted disagreement among other researchers not involved in the study.
PolyPhen-2 predicted 10 of the missense variants to be possibly damaging and 7 to be probably damaging.
When they do not exist, aid is not useful and probably damaging.
PolyPhen-2 categorizes the variants in three groups: benign, possibly damaging, and probably damaging.
PolyPhen also correctly identified 3 as probably damaging (pathogenic), but missed classified the same 2 variants as PMut.
Nonsense variants were assumed probably damaging.
Both SIFT and Polyphen2 predict this variant as probably damaging.
Only one variant was predicted as probably damaging.
Using the PolyPhen analysis tool, the variation was predicted as "probably damaging" to protein function.
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