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The inconsistency between the conclusions obtained under the two pooling probabilities assumptions is the result of the fact that isoform-specific peptides associated with AS events have higher pooling probabilities under the " equal pooling probability" assumption than under the " equal expression" assumption.
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Thus they can be applied to the follow-up researches to remove the adverse initial effects of defining the SOC distribution with some probability assumptions.
A third difficulty stems from the problematic status of the probability assumptions that are required in addition to the basic mechanical laws in order to recover thermodynamic principles within statistical mechanics.
We performed the in silico experiments using two different pooling probability assumptions.
Although neither of the two pooling probability assumptions is truly realistic in a biological sense, the " equal expression" assumption arguably provides the better approximation.
What must that probability assumption look like and how may it be justified?
The status and explanation of the initial probability assumption remains the central puzzle of non-equilibrium statistical mechanics.
It has been generalized in many ways under the uniform probability assumption.
In conventional reliability-based optimum design of structures, the reliability is computed based on the probability assumption, and the design objective and constraint are considered as deterministic ones.
In particular, IBSR provides the lowest MSE and the highest probability of detection in case 1 and 2, which demonstrates the improvement achieved by using the nonidentical Laplace probability assumption.
We can call the assumption corresponding to this the equal probability assumption.
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