And some of these mice hardly developed any mets at all; their lungs looked pristine and uncolonized even two months after the exposure.
It was a Master of the Universe moment as we stared down, jaws adrop, snapping pictures and filling our lungs with pristine air.
Coccini et al. [ 26], in a study on human astrocytes and lung epithelial cells exposed to pristine and moderately and highly functionalized MWCNTs, found higher cytotoxic effects for highly functionalized (hf) NH2-MWCNTs.
As applications of functionalized CNTs linked with therapeutic molecules are still not assayed in man for clinical studies, most publications found in the literature suggested that pristine CNTs could be the source of occupational lung diseases in workers of CNT industries like asbestos pathology previously observed in man [ 45, 60, 62].
We measured the level of oxidative damage to DNA as the premutagenic 8-oxo-7,8-dihydro-2′-deoxyguanosine 8-oxo-7,8-dihydro-2′-deoxyguanosine 8-oxo-7,8-dihydro-2′-deoxyguanosine 8-oxo-7,8-dihydro-2′-deoxyguanosine 8-oxo-7,8-dihydro-2′-deoxyguanosine 8-oxo-7,8-dihydro-2′-deoxyguanosinesolution or corn oil.
In another study exposure of lung A549 cells for 24 hr to -OH functionalized and pristine MWCNTs resulted in a concentration-dependent increase of direct DNA damage [ 49].
Our data are in agreement with studies where the uptake of pristine C60 in nano-aggregated form by MCF10A normal breast epithelial cells [16], lung epithelial adenocarcinoma A549 cells [28], and leukemic monocyte macrophage RAW 264.7 cells [29] was demonstrated.
In the present study, we evaluate and compare the toxicity of industrially produced pristine and -COOH functionalized MWCNTs, studying cytotoxic, genotoxic/oxidative effects and proinflammatory response on two human respiratory cell lines: lung alveolar epithelial cells (A549) and bronchial epithelial cells (BEAS-2B).
So little looked pristine.
