Summary: Computational gene function prediction can serve to focus experimental resources on high-priority experimental tasks.
The following five modules were selected as high priority for experimental manipulation in a factorial design: Normative Feedback; Cognitive Bias Re-training; Self-monitoring and Feedback; Action Planning, and Identity Change.
Determining the genetic basis of rescue – that is, the number and effect size of genetic changes – is essentially an empirical question and has been identified as a priority for experimental work (Gonzalez et al. 2013).
These 2 residues and the other 10 very strong predictions (N72, P78, H158, W174, Y212, M215, H222, C257, N294, and Y302), for which no evidence exists in the literature, should have high priority for experimental vetting to increase understanding of structure/sequence-function relationships for this physiologically important receptor.
Having a theoretical element in your research plan can increase the impact of your work while helping to clarify experimental priorities.
This approach is thus more time-efficient than genomic walking and stable transformation methods, and allows investigators to estimate experimental priorities.
We identified a set of ultra-conserved orthologs within the largest Plasmodium gene family pir, which should be considered as high-priority targets for experimental functional characterization and vaccine development.
Building the model has highlighted priorities for future experimental work, for example the need for experimentalists to work closely with computational modellers in order to generate appropriate quantitative data that is of benefit to systems biology models such as this one.
It is important to note that the sectioning capability depends on the L1 coupling lens (doublet lens, focal length: 200mm): the shorter focal length of L1 provides a larger field of view and less sectioning capability, and a compromise can be selected depending on experimental priorities.
Both approved and clinical targets are potential drug targets, however, approved targets will undoubtedly be on the priority list for further experimental studies.
In its simplest form, the best model in terms of coverage and quality and the complete metadata for a given sequence are made available to the user, giving priority to any available experimental structures.
