There are two aspects of providing priority to buses at intersections: time prioritizing and space prioritizing.
Previous gene prioritization strategies for prioritizing genes in linkage studies evaluated one or more characteristics of the genes, including functional annotation, gene-expression data or sequence-based features (Tranchevent et al., 2011).
In the absence of functional information for most of the identified rAc-DAF-16 linked genes, evidence of differential expression is the most important source for prioritizing future investigations.
We describe here the creation of a new web-accessible gene-gene correlation resource, and demonstrate the power and utility of a large collection of gene expression microarray data for functional gene discovery and for prioritizing genes for mutation analysis within linkage regions.
Using miRNA-specific stem-loop qPCR assays [24] we validated the expression of ten most upregulated miRNAs from the significant list, and also prioritizing miRNAs which appeared in previous studies on pancreatic islets or insulin-secreting beta cell lines such as miR-124, miR-376a, miR-132 and miR-212.
However, the SB transposon mutagenesis strategy allows for a second option: prioritizing mutant mice based on the genes that are disrupted and establishing homozygous mutant lines for expression and diabetes monitoring.
The main objective of this study was to test a novel approach for prioritizing of SNPs from GWAS by combining the results from these studies with publicly available genome-wide expression profiling data from key tissues (i.e. pancreas, adipose tissue, liver and skeletal muscle) of T2DM.
Therefore, a useful approach to prioritizing candidate genes at a locus is to determine if the genes at the locus exhibit variation in expression that is controlled in cis.
However, genome-wide target identification is very useful for identifying regulated pathways or processes, or for prioritizing targets for further analysis (e.g. integrating with other evidence such as ChIP or in situ expression data) or further experiments (e.g. ChIP or perturbation experiments on high-ranking targets).
Prioritizing physical infrastructure.
"Right now it's all about prioritizing," he said.
