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However, the validity of these results has been questioned by the observation that antibodies may induce formation of cell aggregates and cell death is induced upon dispersion of these aggregates prior to the quantification of cell death by flow cytometry.
Media was replaced twice repeatedly prior to the quantification of secreted proteins.
The corresponding membranes of the Transwells are photographed prior to the quantification of fluorescent cancer cells, located at the lower part of the membrane.
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For unambiguous quantification of the amines we oxidized all thiol functions prior to the quantification assay by treatment with hydrogen peroxide for 1 h at 37 °C.
The knowledge of CID-fragmentation pattern of the precursor protonated (or deprotonated) molecule is essentially required prior to the analysis quantification of the desired compounds by LC-MS/MS.
The primers (Invitrogen) were optimized prior to the relative quantification of gene expression.
The strong adsorption phenomenon of AC can be used as an effective preconcentration step prior to the actual voltammetric quantification of the analyte.
Pellets were resuspended in H/S buffer, aliquots were plated on TSA, and grown overnight prior to quantification of the number of intracellular bacteria.
This color change, when initiated using antimony trichloride as the Lewis acid, is known as the Carr Price reaction and was a primary means of retinol detection and quantification prior to the advent of chromatographic methods.
The most important complication to the quantification of H or H-[C] NMR spectra is the use of incorrect prior knowledge on the chemical shifts and scalar couplings.
MPO was removed after reaction with p-nitrophenyl phosphate and prior to quantification of the p-nitrophenolate anion by centrifugation of the solution through a 10000 Da molecular-mass cut-off filter (Pall Life Sciences) at 11200 g for 5 min.
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