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The extract was prepared only prior to the preparation of the FT pellet to maintain its freshness.
Prior to the preparation of the K map, soil map for the area has been generated.
As a consequence, the molecular weight profile of silicone lubricants should be established prior to the preparation of prefilled syringes.
Prior to the preparation of the FIB sections, we tried to remove the grains using a tungsten needle inside the FEG-SEM instrument.
Prior to the preparation of the cremaster muscle, animals were placed on a heating pad and the insertion of a fine polyethylene catheter (PE10, 0.28 mm internal diameter) into the right jugular vein served to apply drugs and fluorescent dyes.
The treated cells were harvested prior to the preparation of cell lysates as described previously [ 27].
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Thirty minutes prior to the preparations of the electron microscopy grids, the ADP myosin samples were incubated in 10 mM imidazole pH 7.0, 10 mM NaCl, 1 mM MgCl2, 1 mM DTT, 3 mM NaN3 and 0.5 mM ADP on ice.
They also served to verify possible ambiguities in the learning documents and, importantly, they served to assess whether students learned from the documents, because we included additional pretests in the tutorials to reveal student knowledge level prior to the preparation phase (assessment of the starting level).
Prior to sequencing, the preparation of the DNA library was performed using a Template Preparation Kit (Pacific Biosciences, Inc., CA) with fragments size targeted at 10 kb.
Prior to beginning the preparation of a radiopharmaceutical, the following checks are recommended: The hot cell air tightness should be tested, verifying that the pressure meets the specifications.
The performance improvement manager recorded time performing the following tasks: 1) prior to the visit: preparation of the clinic note, review of the patient record; 2) during the visit: interaction with the patient with and without the computer, physical examination; and 3) after the visit: documentation of the visit (typing or dictating).
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