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In almost all other processes, sterility of the equipment and medium needs to be ensured prior to the inoculation of the desired microorganisms and ingress of foreign microorganisms prevented throughout the production.
Prior to the inoculation of pre-cultures, each spore suspension (500 μL) was heat-shocked in a water bath for 10 min at 70°C (C. acetobutylicum ATCC 824 and its transformants) or 1 min at 100°C (C. beijerinckii NRRL B593).
Prior to the inoculation of the bacteria, fibronectin was removed and wells were washed and air-dried.
Drug treatment (150 mg/kg i.p). was initiated two days prior to the inoculation of tumor cells and continued daily during the experiment.
Serum samples were collected and the HI titer was not detected in all the chickens prior to the inoculation of the plasmid DNA.
This solution was autoclaved at 121 °C for 20 min prior to the inoculation of 2%% of Streptococcus mutans strain ATCC 25175 (10 cfu/ml), with pH around of 4.0.
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In an intraperitoneal tumour model the alloimmunization of mice with allogeneic P815 tumour cells or splenocytes IP prior to the intraperitoneal inoculation of syngeneic tumour significantly diminished the anti-tumour effects of IL-2 and LAK cell immunotherapy in 7 consecutive experiments.
The assessments were done using sera obtained prior to and following the inoculation of subjects with RV-16, a group A strain.
The flank skin of C57BL/6 mice was depilated and scarified prior to direct inoculation of the skin with 5 × 10 pfu (HSV-2(4674)) or 1 × 10 pfu (HSV-1(17)) in 5 µl volume.
Prior to fungal inoculation of the VIGS treated sorghum plants, approximated 3 cm of the second leaf above the VIGS inoculated leaf was collected from 3 independent plants in triplicates for each condition and used for RNA extraction as previously described.
Prior to the addition of perchlorate and inoculation, the nails were removed from the liquid medium.
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