In group B (n = 4), non-radioactive TAF (3.1 nmol, in PBS) was injected 5 min prior to the injection of [18F]TAF (17.8 ± 1.7 MBq, 1.2 ± 0.1 nmol, in 10% EtOH in PBS); otherwise, the experiments were performed as in group A. Two blocking studies were performed in order to ensure the specificity of the binding at the tumour site.
Hereto a minimal dose of LPS was i.v. injected 30 minutes prior to the injection of TNP-OVA and the animals were subjected to the same protocol as for TNP-OVA only.
However, when estrogen or MK-801 was injected centrally 10 min prior to the injection of the other drug, significant neuroprotection was observed.
GBR12909, fluoxetine, or nisoxetine dissolved in distilled H2O/0.9% NaCl (50 50, v/v, 2 mg/ml) were injected intravenously into rats 60 min prior to the injection of [18F]CFT.
Based on a previous report [ 20], the PWMT and PWTL on the injected side and the contralateral hindpaw were investigated 30 min prior to the injection of BV and 2 hours after the injection.
For the blocking study, a SD rat (n = 3, male, 260 to 280 g; 8 weeks old) was injected with unlabeled MMPIP (1 mg/kg) 30 s prior to the injection of [11C]MMPIP (51 ± 5 MBq, 0.5 nmol).
To investigate this possibility, rats were intraperitoneally (IP) injected with indomethacin, a COX inhibitor, 30 min prior to the injection of visfatin, and their sickness responses were observed.
Bleomycin was injected into the tail vein of nude mice prior to the injection of HLSCs.
In pseudofed experiments, mice were injected with 2 mg kg−1 of glucose intraperitoneally 15 min prior to the injection of F-FDG.
As used in Oklahoma and Florida, midozolam was supposed to render condemned prisoners insensate prior to the injection of two other drugs, which caused general paralysis and stopped the heart (the constitutionality of the latter two drugs was not at issue).
The antibody was given 72 h prior to the injection of [18F]TAF.
