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In the present study, we treated the cartilages with collagenase prior to the extraction of total RNA, because it is difficult to extract the total RNA from hard and ECM-rich tissues such as bone and cartilage [ 8, 34, 35].
An internal standard was added, prior to the extraction of total lipids (17), for quantification of TG being extracted from cells and cell medium.
To demonstrate the necessity of the thermalization step prior to the extraction of polarized solid beads, a series of ex situ dissolution experiments were performed.
Wise choices for scanning parameters and consideration of instrument specifics will ultimately increase data reliability and reduce the need for complex preprocessing mechanisms prior to the extraction of expression information.
Selective sulfation-roast-water leach process of a complex, chromite-rich Cu-Ni-PGM ore to dissolve water-soluble base metal sulfates contained therein was investigated as an alternative process to remove base metals prior to the extraction of precious metals.
Many studies apply pretreatment techniques prior to the extraction of the nucleic acids from the fixed tissues.
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Prior to the extraction, 50 μl of saturated NaCl solution was added and the carotenoids extracted with one volume of dichloromethane.
Prior to the extraction, 1 g of powdered propolis sample was homogenized and mixed with 10 mL of 80% of ethanol/water and kept at 70°C for 1 h.
The pH was neutralized prior to the extraction and purification of the components.
Prior to the extraction, 100 ng of the standard p-terfenil-d14 was added to the sample to comprise the aromatic fraction.
11 Therefore, the pre-extraction PT-INR values we utilised may not have accurately reflected the coagulation status immediately prior to the extraction, skewing the results of our analyses.
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