Neurons from neonatal animals (P1) were isolated and plated from individual pups; genotyping was done prior to the day of experiment.
Based on the same interpolation methods, we calculated estimates of short-term exposure to O3 based on the moving averages of 8-hr maximum O3 concentrations 1 30 days prior to the day of blood collection.
Each village was visited two days prior to the day of survey by staff of the Helminth Control Laboratory Unguja (HCLU) who would liaise with the local village leader (shehia) for community sensitization and assistance in open enrolment of future patient volunteers (> 16 years of age) for the day of survey.
Eligible subjects were healthy Japanese males aged between 20 and 39 years with a body mass index (BMI) between 18.5 and 24.9 kg/m and who were considered healthy, based on the results of a screening examination (medical examination, vital signs, and ECG examination) for determination of eligibility performed between 28 and 3 days prior to the day of administration.
Models were fitted to daily temperature and rainfall of the day of death and of mean temperature and rainfall up by 21 days prior to the day of death (lags 0 21) to identify the effects of longer periods with high or low temperatures or high or low rainfall.
The time-varying model was adjusted for each component of SOFA score on all days in the ICU prior to the day of first event.
We contacted participants 6 weeks prior to the day of testing to establish the two "typical" working days separated by 1 day (preferably Tuesday/Thursday to avoid sampling on the beginning and end of the work week) on which they would provide the at-home saliva samples.
Following detailed instructions about the timing and conditions of saliva collection, participants completed two nonconsecutive days of saliva collection at home prior to the day of testing and then sent the saliva samples overnight mail to the University of California, Davis to be assayed under the direction of SM.
Mean postoperative LOS (excluding LOS prior to the day of surgery) for the 105 patients in the preimplementation period was 7.5 days (SD 3.0).
Mice were fasted overnight prior to the day of sacrifice.
