The mixture was stirring for an additional 5 min prior to the addition of 2 mL of 1 wt % HAuCl4.
Mineral salt medium was sterilized by autoclaving prior to the addition of chlorpyrifos.
Both malate and pyruvate (PM) were added prior to the addition of cells.
Prior to the addition of perchlorate and inoculation, the nails were removed from the liquid medium.
Blanks were prepared in the same manner, except ammonium sulphate which was added prior to the addition of crude extract.
Prior to the addition of deuterium, Abl was allowed to equilibrate with each drug.
DU145 cells were treated with TSA and FGF19 prior to the addition of TNFα.
The potential inhibitors were added 1 h prior to the addition of the anticancer drugs.
Prior to the addition of MutL, the free beads were stringently removed by extensive washing [17].
Cell lines were treated for one hour prior to the addition of CXCL8, at a concentration of 0.022 µM.
Cytochalasin B treatment of APCs prior to the addition of liposomal antigen in the culture had little effect.
