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Damour and Oansan [ 20] noted that educational efforts should be marshalled early in the curriculum, prior to the solidifying of professional identities and the formation of stereotypes.
Callus was induced on 0.5-cm long hypocotyl explants excised from sterile bean seedlings (Phaseolus vulgaris cv Tendergreen) on the basal medium of Murashige and Skoog [19] supplemented with 0.5 mg/l 2,4-dichlorophenoxyacetic acid, 0.1 mg/l kinetin and 30 g/l sucrose, with the pH set to 5.6 prior to autoclaving and solidified with 7 g/l purified agar (Oxoid Ltd. UK).
Fixed cells were mounted using Fluoromount G supplemented with 25 mg/ml DABCO (1,4 diazabicyclo[2.2.2]octane) (Sigma-Aldrich) and allowed to solidify overnight prior to imaging.
Coverslips were then mounted on slides using Fluoromount G supplemented with 25 mg/ml DABCO (1,4 diazabicyclo[2.2.2]octane) (Sigma-Aldrich) and allowed to solidify overnight prior to imaging.
Ask your healthcare provider whether there are additional tests necessary to solidify your diagnosis prior to beginning aggressive or targeted treatment for Inflammatory Bowel Disease.
This basal layer was allowed to solidify for 10 minutes at 4°C prior to reequilibrating at room temperature for 30 minutes.
Briefly, melted gelatin is added to the TNFS which is incubated at room temperature and then cooled to solidify and encase the detaching cell sheet prior to full sheet detachment.
Prior to injury, 300 μl of dissolved VAS2870, PEG-SOD or vehicle were applied perivascularly to the artery and allowed to solidify for 30 min, after which balloon injury was performed.
To solidify sales?
If necessary, refrigerate to solidify.
emerged to solidify this tendency.
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