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Antarctic Phosphatase was heat inactivated (65°C for 10 min) prior to separating the DNA on 1 % TAE agarose gels and gel extraction (performed as already described).
Samples were analyzed by dissolving in formamide loading buffer prior to separating by denaturing PAGE.
The hydrolysis aliquots at 10%and20%0% (w/w) concentrations were diluted tenfold immediately prior to separating the solid and liquid phases (with centrifugation at 3000 rpm for 10 min) when sampling in order to minimize the measurement error introduced by the high amount of insoluble, leading to overestimation of the yield [ 24].
With the Veteran's Opportunity to Work (VOW) Act of 2011, it is now the Law that every transitioning military member attend this training prior to separating active duty.
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Mature capsules were dried at 60 °C for 3 days prior to separate weighing of capsule and seed masses.
For foci counting, the gain was reduced on Cdc45-injected nuclei prior to separate image acquisition.
The exponential nature of inflation wipes out any information that occurred prior to that, separating it from anything we can observe by, well, inflating it beyond the portion of our Universe that we can observe.
For identifying the region of chromosome 22 retained in BCNU-resistant cells, BAC DNAs were individually labeled and tested prior to being separated into one of 4 groups.
Bioluminescent imaging was conducted using the Xenogen Ivis Spectrum (Caliper Life Sciences, Hopkinton, MA) to ensure that the animals had roughly equivalent tumor burdens prior to being separated into treatment groups.
Prior to 2001, separated people were instructed to record themselves as married but information collected elsewhere in the census (particularly questions on relationships in the household) means that is possible to identify separated people in earlier censuses who here have been grouped with the divorced.
However, it is also apparent when sequences from humans and the more closely related chimpanzee are compared, that duplication events have taken place prior to these species separating.
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CEO of Professional Science Editing for Scientists @ prosciediting.com