The first involved 1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine (DiI) labelling of the wound bed, prior to replacing the skin graft, to allow tracking of host cells into the grafts.
The agreement between two disease assessment approaches is important to know prior to replacing or interchanging the use of an established method with a recently developed method of measurement.
Christie was a first-team coach and lottery manager prior to replacing Brewster, initially on a caretaker basis, and continues now as the club's head of youth development.
The verified model was then employed to conduct a parametric study considering important micro-tunneling design parameters, such as depth and diameter of the tunnel, in situ stress ratio (Ko), and the time lapse prior to replacing LFs with permanent cement grout around the tunnel.
The transfected COS7 cells were cultured in DMEM supplemented with 10% heat-inactivated FBS at 37°C and 5% CO2 for 16 24 hours prior to replacing the growth medium with serum-free DMEM.
The swab will air dry for 1-2 minutes prior to replacing it into its container.
In contrast, preculturing of hESCs for 24 h in MCM, prior to replacing MCM with MCM + 10% old mouse sera was done for embryonic microniche experiments (Fig. 3).
For 1-day galactose experiments, cells were maintained in glucose-supplemented media for 4 to 5 days prior to replacing with fresh glucose or galactose media, thereby minimizing differences in total culture duration.
Another player Grayson will hope he can now keep is Gallagher, who is on loan from Sheffield United and made a telling contribution prior to being replaced on 37 minutes after taking a kick to the calf.
The CAPRA score and similar multivariable stratification systems are posed to replace prior older and less accurate risk group categories.
