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Prior to processing with the cleaner4d program, the data were square cosine weighted in the directly acquired dimension and zero-filled to 2 k points using NMRPipe/NMRDraw 3.038.
All images were acquired with an AxioObserverZ1 equipped with a Colibri-LED-system (365/470/590 nm) and an AxioCam HRm, and with either an 63x oil Plan-Apochromat (fixed cells) or an 40x oil EC Plan-Neofluar objective (live cells) prior to processing with the AxioVision rel 4.7 software (Zeiss).
Total RNA samples were shipped on dry ice to the laboratories at Miltenyi Biotec (Auburn, CA), where they were quality-checked prior to processing with the Agilent 2100 Bioanalyzer platform (Agilent Technologies, Santa Clara, CA).
Blood collected from each fish in set 2 was spiked with 1.5 pmol of the synthetic steroid ethinyl estradiol prior to processing to confirm the compatibility of the method with an internal standard.
In cells fixed and permeabilised with Triton-X-100 prior to processing for anti-myc immunofluorescence, both mCherry signal and anti-myc immunoreactivity were detectable (Figure 5C, mCh-CaBP7-myc and Figure 5G, mCh-CaBP8-myc).
The chromatographic data of all test ESTs was cleaned with the program phred prior to processing by one of the trimming programs (see methods).
Prior to processing, a job must be loaded (setup) by a single server onto the relevant machine.
Prior to processing a force-volume image, it is necessary to set the values of the algorithm parameters.
Transfer of bacteria from root surfaces was avoided by coating roots with agar prior to processing.
Samples were transferred to a -70°C freezer for holding prior to processing.
Larvae were treated with chloroquine (2.5 μM) for 14 h prior to processing.
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