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The plots at each site were established on commercial forest land in existing plantations that had been re-spaced more than 10 years prior to plot establishment.
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A porosity cut-off of 15%% was applied prior to plotting data.
Data from all individuals for a given enrichment platform was pooled prior to plotting the density function.
Gene expression fold changes were log2 transformed prior to plotting.
To correct for the frequency of errors inherent to sequencing, mismatch values for each position of 15 76 nt RNAs in wild-type plants were subtracted prior to plotting the data.
In stands established prior to 1990, plots of 0.06 ha were installed during 2010 on an 8 km grid.
In stands established prior to 1990, plots of 0.06 ha were installed during 2010 on a complete 8 km grid.
Prior to analysis, a plot of ordered probe set intensities was produced to establish a suitable threshold above which intensities could be considered true signals.
Future studies may seek to derive accurate footprint estimates prior to establishing sample plots to ensure they are representative of the broader site conditions.
In both years, the percent cover of A. petiolata, other plants, and bare ground was estimated from each plot prior to weeding, and for A. petiolata removal plots, the weeded biomass was collected, dried, and weighed.
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