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Cells were rinsed twice with medium with 0.1% serum prior to labelling.
Frozen sections (5 7 µm) of mouse dorsal and tail skin were fixed for 10 minutes in 4% paraformaldehyde prior to labelling.
RNA was not amplified prior to labelling.
Prior to labelling, bacterial RNA was polyadenylated and reverse transcribed.
Prior to labelling, grids were washed in ice-cold distilled water (×3) followed by PBS at room temperature.
The clones were sequence verified and the corresponding inserts were isolated using appropriate restriction analysis and purification prior to labelling.
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As FDG is a glucose analogue, the ASCs were incubated in glucose-free medium for 30 min prior to labeling with FDG.
Cadmium treated cells were supplemented with 50 mM Cd2+ sulfate for one hour prior to labeling.
Yeast and fungal pathogens were cultured and grown prior to labeling with fluorescent dye.
As a control, root sections were exposed to 2 mg ml−1 cellulase for 16 h prior to labeling.
For competition assays, 100-fold excess of unlabeled NF-κB probe or its mutant probe was added 20 min prior to labeled probe.
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