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To verify the specificity of PI3K-C2 immunoreactivity, blocking studies were performed by pre-incubation of each antibody with the protein fragment used to raise it prior to its application in IHC.
This study recommends that the coconut fiber undergo treatment prior to its application in concrete to protect it against degradation or that it be replaced with a non-corrosive fiber.
In this paper the effect of aliasing is viewed as a 3 dB frequency warping and a pre-warping step prior to its application is proposed to reduce it, thereby making the technique appropriate from the viewpoint of frequency domain also.
To verify the specificity of the CTCF immunoreactivity, blocking studies were performed by preincubation of the primary antibody (at different dilutions) for 2 h at room temperature with the protein fragment used to raise it (Santa Cruz Biotechnology, sc-5916 P) prior to its application to the tissues according to the supplier data sheet protocol.
Consequently, it is important to properly calibrate this uncertainty prior to its application in the geotechnical analysis to ensure the integrity of the final design.
However, limited convergence is an inherent difficulty associated with the series and needs to be addressed rigorously, prior to its application to a physical system.
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The model was calibrated by our experimental data with a reasonable agreement prior to its applications.
This FL approach was then compared to the conventional 7Q10 approach for low flow selections prior to its applications, using the USGS flow data from the freshwater environment (Big Sunflower River, Mississippi) as well as from the estuarine environment (St. Johns River, Florida).
The waste composition should however be determined prior to its land application as plants vary in their nutrient requirements.
Although promising, direct cardiac reprogramming is still in its fledging phase, and numerous barriers have to be overcome prior to its clinical application.
However, due to the volatility and delicate properties of volatile flavor compounds, encapsulation of flavor compounds prior to its final application as food ingredients is often done [3].
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