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Fibroblasts were inoculated on collagen sponge and cultured for 1 week prior to inoculation of ESCs.
No B. thetaiotaomicron SSU rRNA was detected in fermenter samples prior to inoculation with strain BTX.
During experiments with different G. sulfurreducens inoculum concentrations, the concentrated inoculum solution was diluted using fresh co-culture medium prior to inoculation.
The protease inhibitors were added only once, 1 hr prior to inoculation with virus.
Plates were left to cool in a laminar flow hood for 24 hours prior to inoculation.
Hydrolysates were always filter sterilized (0.2 μm, Millipore, USA) prior to inoculation.
Bacteria were washed twice in sterile anoxic 0.1 M NaCl prior to inoculation into experimental media.
The incision sites were healed for at least 7 days prior to inoculation with bacteria.
Sterile experimental media were allowed to equilibrate in the anaerobic chamber at 30°C for 24 h prior to inoculation.
Headspace monitoring of these cultures was done prior to inoculation to ensure that they were not in latency phase.
The POME was adjusted to the desired pH using 5 M H2SO4 or NaOH prior to inoculation.
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