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Following incubation for 20 min, the samples were washed (to simulate removal of compound for clinical use prior to, for example, IUI) and incubated for up to 300 min. There was still a significantly higher total and progressively motility compared with controls at the end of incubation with each compound (Table II).
Zebrafish embryos were fixed according to their developmental stage (Additional file 2), in 4% PFA in PBS (137 mM NaCl, 2.7 mM KCl, 10 mM Na2HPO4, 1.8 mM KH2PO4, pH 7) at RT prior to (for embryos younger than 24 hpf), or after (for 24-hpf embryos and older) hand dechorionation.
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The manuscript was sent to Amgen for review prior to submission for publication.
The manuscript was approved by SEER-Medicare for anonymity prior to submission for publication.
Posture changing frequency was recorded for 2 h prior to and for the duration of farrowing.
Prior to Students For Liberty, he worked for the Cato Institute on student programs.
He reported taking non-steroidal anti-inflammatories for 1 week prior to admission for an epicondylitis.
Prior to working for Obama, Zichal worked for then-Senator John Kerry.
The 3,007-square-foot 3,007-square-foot 3,007-square-footip prior to listing for sale in May for $8.795 million.
OVCAR-3 cells were transfected with siRNA for 48 h prior to examination for cell adhesion.
Pathogen-free nymphs were allowed to feed for 18 h prior to dissection for salivary glands.
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