Sentence examples for prior to flash from inspiring English sources

Crystals that appeared were transferred to cryoprotectant solution composed of mother liquors supplemented with 5%% (v/v) ethylene glycol prior to flash cooling in liquid nitrogen.

Prior to flash freezing, crystals were transferred for 10 20 s to a 10 µl drop of reservoir solution containing 10% (v/v) glycerol for cryo-protection.

The crystals (P3221 space group; a = 59.38 Å, b = 59.38 Å, c = 95.76 Å) were cryo-protected by immersion in paratone-N (Hampton Research) prior to flash freezing.

Crystals grew overnight and were cryo-protected with 20% glycerol in drop mother liquor prior to flash freezing in liquid nitrogen.

Prior to flash freezing, the crystals were briefly soaked in mother liquor supplemented with 20% ethylene glycol.

Crystals were cryo-protected by addition of 20% (w/v) ethylene glycol prior to flash cooling in liquid nitrogen.

For data collection, the AVR2-b crystal was cryoprotected with 20% glycerol (v/v) and 2 M lithium sulfate just prior to flash-freezing in a 100 K nitrogen stream (Oxford Cryostream).

Crystals of SpnP complexed with TDP were obtained by supplying 20 mM TDP (final concentration) to the crystallization drop and incubating it for 1 h prior to flash-freezing.

These crystals were soaked in 0.1 M Tris, 0.4 M ammonium acetate, 12−13% PEG 10K, 5 mM DTT, pH 7.0−8.0 supplemented with increasing concentrations of glycerol (5−30%, in 5% increments) prior to flash-freezing in the presence of 30% glycerol and data collection at the 8.2.2 beamline at the Advanced Light Source.

A number of different strategies for cryoprotection were attempted [27], [28], such as quick soaking in mother liquor supplemented with glycerol, MPD or glucose, serial transfer of crystals to mother liquor containing increasing concentrations of the selected cryoprotectant, crystal dehydration [29] prior to flash-cooling and annealing [27].

Recombinant Mtb-BirA was crystallized at room temperature, and diffraction data was collected at 295 K as well as at 120 K. Transfer of crystals to paraffin and paratone-N oil (cryoprotectants) prior to flash-freezing induced lattice shrinkage and enhancement in the resolution of the X-ray diffraction data.