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The use of PrepStrips™ to flatten prior tissue prior to LCM was helpful for both MA and MG sections.
Mice were kept on the respective diets for 3 weeks prior tissue harvest as described previously [ 15].
One of the limitations of our approach was that, to exclude effects from prior tissue sampling, pre- and postbiopsy samples were collected from different legs.
The primary outcomes were the rate of malignancy of nGGOs among patients who underwent surgical resection without prior tissue diagnosis, and the clinical and radiological predictors of malignancy.
Owing to the emergent circumstances, large tumor size with necrosis, and no prior tissue diagnosis, nodal sampling was performed; lymph nodes were grossly nonenlarged.
Our TU-tagging experiment sought to purify transcripts from auditory, vestibular and lateral-line hair cells from whole larvae without any prior tissue enrichment.
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After being anesthetized, the mice were perfused with saline followed by 4% paraformaldehyde prior tissues dissection.
In both microarray studies, blood was not perfused from tissues prior to tissue collection and it is known that leukocyctes express genes during the early wound-healing phase of spinal cord and limb regeneration.
For zymography studies, prior to tissue collection, mice were perfused with 10 ml PBS and the tissues were frozen in liquid nitrogen and stored at -70°C until use.
The tissues were embedded immediately in OCT compound, frozen in liquid nitrogen, and stored at -70°C prior to tissue sectioning and immunohistochemical staining.
Consistent with prior reports, tissue type was found to have a major impact on a transcriptome such that data containing multiple tissues yielded no qualified classifiers.
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