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The sample solutions for cation analysis were stored in the fridge prior the analysis.
Samples of 20 mg were dissolved in d4-methanol prior the analysis.
Since a proper alignment of the molecules prior the analysis is crucial to the successful application of these models in further studies, the molecules were aligned based on their pharmacophore features.
Propidium iodide to a final concentration of 50 µg/ml was added to the nuclei suspension and incubated 5 min prior the analysis.
The nuclear sequences corresponding to the 21 first amino acids in the chloroplast clpP1 gene of Spinacia were excluded prior the analysis, because the alignment responded strongly even to small changes in the parameters.
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These interferences must be removed prior to the analysis.
The samples were stored at −20 °C prior to the analysis.
All time series were deseasonalized prior to the analysis.
Samples were centrifuged and diluted (1 4) prior to the analysis.
Finally, the milled powder was packed in the airtight container prior to the analysis.
The film was then incubated prior to the analysis with densitometry software.
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