Sentence examples for prior sorting from inspiring English sources

Cell cycle analysis and AnnexinV staining were performed as previously described [66] and without prior sorting.

The Gap1-152 newly synthesized on proline medium also accumulates in the class E compartment of vps27Δ mutant cells (Fig. 5C), indicating that its delivery to the vacuole involves its prior sorting into the MVB pathway, as expected.

Delivery of Gap1 into the vacuolar lumen involves its prior sorting into vesicles budding into the lumen of the late endosome via the multivesicular body (MVB) pathway [9], [29].

To determine the expression of putative cancer stem cell markers in the spheroids, we plated A1.8 and RP.1 cell lines without prior sorting as single cells/well in low-binding 96-well plates.

Prior to sorting, the cytometer was cleaned thoroughly with bleach.

(B) Separated cells were stored in EDTA for up to 7 days prior to sorting.

(A) The influence of formaldehyde (HCHO -based and EDTA-based additives on RNA integrity, wHCHO -basedlood wandstored at ambient temperadditivesat 4°C fon up to 7 days pRNAr to sortintegrity

EDTA-admixtures resulted in constantly high RIN values for the cell preparations up to 7 days of storage at 4°C. (B) All five tested cell types (N = 5 each) showed a good total RNA quality when stored in the EDTA blood collection tubes at 4°C for up to 7 days prior to sorting.

Cells were then filtered through a 40 µm nylon mesh cell strainer prior to sorting.

Lineage+ cells were depleted prior to sorting using the EasySep Cell Biotin Selection Kit (StemCell Technologies Inc).

Cells were resuspended in PBS 0.5% BSA and passed through nylon mesh prior to sorting.