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The method used starts with a prior sequence model.
We measure this by computing the mutual information between a prior sequence and the PSR.
Admissibility is always relativized to the prior sequence: the order of introduction matters.
The presented genome walking protocol could be applied to any microbial genome and requires only 100 150 bp of prior sequence information.
We have developed a tea plant oligonucleotide microarray that can be used to analyze gene expression in the shoots of different tea plant clones without prior sequence knowledge.
The present study is the first of its kind to sequence TST exomes captured by use of cDNA molecules for SNPs found in the coding region without any prior sequence information of targeted molecules.
It obviates the need for an assumption to guild testing suspected viral pathogens with multiple viral-specific primers to amplify targets present in a sample, thus does not require prior sequence knowledge of the viral agent for primer design.
Sequence-independent amplification methods (Wang et al. 2002; Berthet et al. 2008; Chen and Wang 2012) appear to be attractive alternatives to amplify diverse viruses for downstream applications including microarray analysis in that they do not require the prior sequence information of viral pathogens to guide to design virus-specific primers for amplification.
Another factor potentially obscuring prior sequence analyses is the unusually high GC content of LKB1 exonic regions.
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Most importantly, the proposed methods can make principled probabilistic inference from multiple evidence sources, such as, multiple statistical models (motifs) of the TFs, evolutionary conservation, regulatory potential, CpG islands, nucleosome positioning, DNase hypersensitive sites, ChIP-chip binding segments and other (prior) sequence-based biological knowledge.
To the best of the authors' knowledge, only two prior sequence-analysis-based contributions perform similar contrasting analysis, but they focused on identification of β-aggregating stretches (Tartagia et al. 2005) or contrasted just four prion proteins (Pappalardo et al. 2007).
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