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Sheep genotypes were from lines with 10 years prior selection for low (resistant, R) or high (susceptible, S) faecal worm egg count (WEC) following H. contortus infection.
General combining ability (GCA) estimates for seven landrace-derived populations/varieties, derived from multi-environment tests in arid zone environments, indicated that selection history played a large role in determining GCA for both biomass and HI, with prior selection (for grain yield) favoring GCA for HI at the expense of GCA for biomass.
A large number of individual water-soluble metabolites and phospholipids were quantified without prior selection for specific metabolic pathways.
Like other 'omics' (e.g. genomics, proteomics), metabolomics has the benefit of simultaneously providing a broad overview of molecular processes without prior selection for specific pathways [25].
EXPRSS library preparation uses total RNA and unlike NlaIII-DGE no prior selection for poly(A) RNA was performed.
Latent phenotypes may also exist in RNA, which can form multiple secondary structures through thermal motion [ 6, 7], as well as in metabolic networks of chemical reactions, which can be viable on carbon sources without prior selection for such viability [ 8, 9].
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The specific interest of this work was first focusing on global gene expression regulation without prior selection of transcripts for association with a particular trait, and second using a family structured population that enabled genetic analysis.
32 The goal of genome-wide association studies (GWAS) is to identify genetic variants associated with complex phenotypes without the need for prior selection of candidate loci or genes.
Our approach is also likely to be free of biases, as there is no prior selection of sequences to test for enhancer activity.
For example, prior selection of probes in the arrays biases the possible set of transcripts that can be detected; unspecific hybridization cannot be completely eliminated; the differential efficiency of probes makes it impossible to compare the expression of different genes in the same sample, etc.
Building of a thematic core collection was here defined by prior selection of accessions which are diverse for the trait of interest, and then by pairwise genetic distances, estimated by DNA polymorphism analysis at molecular marker loci.
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