R free = ∑T||F o F c||/eT|F o|, where T is a test data set of 5%% of the total reflections randomly chosen and set aside prior to refinement.
The initial MR 2Fobs−Fcalc map prior to refinement is presented in Figure 6.
The H1061N mutation was introduced in Coot [ 34] prior to refinement.
Finally, we subjected the initial resulting maps (prior to refinement) to auto ligand find and auto build.
In order to reduce model bias, we also calculated an omit difference map from a model refined at 3200 K without the use of NCS restraints, from which a whole α-helix containing the H1061N residue was omitted prior to refinement.
Our experiment results suggest significant improvements in individual accuracy prior to optional refinements.
The survey instrument performed well in both survivor and caregiver samples and will require only minor refinements prior to use in further studies.
Then, multiple rounds of inputs were solicited from the entire task force with several subsequent rounds of revisions and refinements prior to submission.
The prototype questionnaire was pretested using cognitive interviews in a purposive sample of myeloma patients with subsequent refinements prior to field testing.
We have found that this design does not produce enough plastic strain per RCS cycle for effective grain refinement prior to the formation and growth of fatigue cracks.
