Sentence examples for prior purification from inspiring English sources
Moreover, even non-stabilized receptors can be analyzed and their prior purification is not necessary for the formation of nanodisc complexes.
SPR biosensors allow the real-time detection and monitoring of the binding between an immobilized protein and its soluble ligand without the need for prior purification and labeling of the mobile analyte.
Epidemiological studies have examined breast cancer risk in relation to sex hormone concentrations measured by different methods: "extraction" immunoassays (with prior purification by organic solvent extraction, with or without column chromatography), "direct" immunoassays (no prior extraction or column chromatography), and more recently with mass spectrometry-based assays.
To investigate the role of DES and the prior treatment with KMnO4 further, pristine graphene flakes were treated directly with some types of DES without prior purification.
In order to exclude the effect of CD56 antibody binding during purification, we also compared the IFNα production capacity of CD56+ and CD56− DCs by intracellular staining without prior purification.
Protein biotinylation and immobilization on a streptavidin-coated surface were also achieved in a cell lysate without prior purification of the target protein.
The linearized plasmids were made blunt ended with Klenow enzyme (New England Biolabs, Beverly, MA) and dephosphorylated by calf intestinal alkaline phosphatase (New England Biolabs), prior to purification with the Qiaquick PCR Purification Kit (QIAGEN, Valencia, CA).
PCR reactions were performed in triplicate and pooled prior to purification.
On the left are crystals prior to purification, on the right are the same nanocrystals after impurities have been removed.
Regardless of product source, removal of cells, cellular debris, aggregates and precipitated material is usually necessary prior to purification.
For KatA1-49 the pellet containing the insoluble fraction was dissolved in binding buffer with 8 M urea and proteins were then refolded by dialysis against binding buffer prior to purification.
