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Zhang and Gerstein followed a prior protocol in distinguishing between two sets of cytochrome c pseudogenes.
Quantitative SMN mRNA levels were performed with slight modifications to the prior protocol [36].
16 17 Key elements are a prior protocol, clearly defined objective outcomes, and transparent sequential reporting of cases, showing when changes in indication or technique are made.
The latter patient had concurrent estrogen use and a prior history of a TEE (deep vein thrombosis [DVT]); the patient had been enrolled under a prior protocol amendment that excluded patients with prior TEEs.
Assuming · N−1 = 0.62 for A. nobilis, hatchery-wide required for Nb = 74 would be 120 brood fish, 40% fewer than stipulated in prior protocol (Bartley et al. 1995; see also Table S2).
To develop the assay in this study, we adapted the prior protocol for the manual PR assay [ 4] in 96-well plates and modified the readout by treating cells with Hoechst 33342 for 20 minutes, washing with PBS, and sealing plates with aluminum foil prior to automated image acquisition (Supp. Note 1).
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In this paper, we propose a cooperative private searching (COPS) protocol that provides the same privacy protections as prior protocols, but with much lower overhead.
Our study has shown substantial agreement between Cochrane reviews and prior protocols over the last few years but also highlights a concern about a previously unreported source of bias.
Per our prior protocols, the microglial cell line EOC 2 was obtained from American Type Culture Collection (ATTC, Manassas, VA).
Per our prior protocols [ 3, 4, 34], β-amyloid (Aβ1-42) (Invitrogen, Carlsbad, CA) was dissolved in PBS at a concentration of 100 μM.
First, in the presence of SU5416 but in the absence of hypoxia, chronic pulmonary expression of miR-210 in WT mice was achieved by serial intrapharyngeal injections of liposomally encapsulated miR-210 oligonucleotide mimics, as adapted from prior protocols (Bertero et al, 2014) (Fig 4A).
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