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None of the prior membrane-targeting studies, however, had the temporal resolution to monitor non-equilibrium differences between membrane-bound and cytosolic GECI signals, nor did any investigate GECI signaling of calcium influx mediated by action potential/voltage-gated calcium channel activity.

By analogy, BAK assembled into high-molecular-weight complexes prior to membrane insertion (membrane anchored conformation; Figure S7i) and consequently could be labeled by IASD.

We showed that EM permeabilization occurred prior to membrane rupture and membrane shedding from activated gametocytes (Fig.  7A).

These membranes exhibit a pervaporation separation performance surpassing most prior polymeric membranes and inorganic ceramic membranes for isopropanol dehydration.

Essentially identical results are obtained with concanamycin A added to preformed membranes as a concentrated solution in DMSO, or mixed with lipid in organic solvent prior to membrane formation.

In the 'in-out' pathway, the TMD fully spans the channel prior to membrane integration; a trajectory thus exhibits this pathway if a TMD visits IS3 on the way to membrane integration without visiting IS4.

Prior to membrane preparation, pellets were stored at − 80 °C.

Prior to membrane separation, the mince was subjected to washing as previously described.

We performed an extensive computational study to obtain insight in the molecular mechanisms that take place prior to membrane fusion.

Prior to membrane synthesis, the supports were cleaned in boiling water for 1 2 h to remove dirt and loose particles from their pores.

Prior to membrane adsorption, a monolayer of β-Tg was first self-assembled at the Au(1 1 1) surface to increase the hydrophilicity of the electrode surface.