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The soil samples were analysed individually, whereas the plant samples (blocks of 30?×?30 cm) were pooled into plant families prior further processing to keep the sample size manageable to allow a parallel DNA-based and microscopic study.
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Prior to further processing, solutions resulting from either acidic or carbonate leaching must be clarified.
Size reduction is the minimum required pretreatment step prior to further processing.
The product is captured from the disrupted cells and cellular debris is removed prior to further processing.
Extracted RNA samples were then sent to Genoscope where, prior to further processing, a DNase treatment using Turbo DNA-free kit was performed as described at the end of Section 2.1.
This revealed one outlier replicate from the entire sample set (Supplementary Fig. 9), which was excluded prior to further processing to optimally assess stratification between the different cellular genotypes.
This equipment is expected to improve drying of challenging industrial sludge materials when used as a pretreatment method prior to further processing.
Fermentation broths are commonly evaporated after harvest to increase the concentration of non-volatile products or byproducts by the economical removal of large amounts of water prior to further processing and purification to finished products.
The recall was issued after FSIS determined there was an association between non-intact steaks (blade-tenderized prior to further processing) and illnesses in Colorado, Iowa, Kansas, Michigan, South Dakota and Washington.
Media supernatant was collected on days 2 and 4 of differentiation and stored at 4°C prior to further processing.
Tissues and swabs were frozen at −70°C prior to further processing for both DNA analysis and virus isolation (see below).
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