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Second, he says that European integration will continue because it will be in the interests of members to do more things together; and that the successful introduction of new members will require some prior further integration of decision-making in EU institutions.These, taken with the centralising effect of EMU, point to more and more power being transferred to Brussels.
Purity was controlled by SDS-PAGE prior further analysis and application in biotransformation.
Prior further analyses, the raw ranks were transformed as follows: each value was divided by the number of evaluated species (40) and square-root arcsin transformed.
A Shapiro-Wilk normality test, with a p = 0.05 rejection value, was used to test normal distribution of data prior further analysis.
Prior further evaluation, the data was additionally normalized to definite starting concentration in order to remove the errors introduces due to uneven sample quantities.
The soil samples were analysed individually, whereas the plant samples (blocks of 30?×?30 cm) were pooled into plant families prior further processing to keep the sample size manageable to allow a parallel DNA-based and microscopic study.
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Using priors further increased likelihood and gave many new time-delayed interactions.
dsRNA products were stored at −80 °C prior to further use.
Dismantling allows components to be separated prior to further treatment.
In all cases expression was allowed to proceed for 48 h prior to further experimental manipulations.
Stable clones were analyzed by qRT-PCR for silencing of viral transgenes prior to further experimental procedures.
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