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The only difference with respect to methods is that three experiments were performed prior to transfection of the MRMT-1 cells line, and this study and one of the prior experiments were conducted after transfection of the MRMT-1 cells line.
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Written informed consent was obtained from all subjects prior to testing, and experiments were conducted according to the Declaration of Helsinki.
Prior to kinetic studies, experiments were conducted to determine enzyme concentrations, dNTP concentrations, and times for which product accumulation was linear as a function of time.
Prior to DNA extraction initial experiments were conducted to determine the most suitable DNA extraction method.
Fifteen male Long-Evans rats (8 to 10 weeks old; Charles-River) were housed individually and had free access to food and water prior to behavioural testing, and experiments were conducted in accordance with the guidelines of the Canadian Council on Animal Care.
All hESC and hIPS experiments were conducted with prior approval from the UCLA Embryonic Stem Cell Research Oversight Committee.
In addition, prior to H12-NEC adsorption, IRAS experiments were conducted to monitor the cleanliness of the surface by CO adsorption experiments at 100 and 300 K.
Prior to initiating transformation procedures, preliminary experiments were conducted to examine the effect of explant types, combinations of plant growth regulators, antibiotic concentrations, and culture conditions.
And all the animal experiments were conducted with the prior approval of Animal Experimental Ethics Committee of the local institution.
All animal experiments were conducted after getting prior permission from the Institutional Animal Ethics Committee and as per the instructions prescribed by the Committee for the Purpose of Control of Supervision of Experiments on Animal (CPCSEA), Ministry of Environment and Forest, Government of India.
Prior to the transduction experiments described below, preliminary experiments were conducted in order to identify the most appropriate time points for detailed analysis.
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