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Fitting SAXIS2 and τe relies on prior characterization of the overall molecular tumbling.
Prior characterization of the Hmx1 dm/dm mouse identified a microphthalmia phenotype in adults but did not quantify the reduction in eye size (Munroe et al., 2009).
The single-proline αSynuclein mutant A56P as well as the triple-proline mutant A30P/A56P/A76P (TP) showed drastically reduced propensity to form proteinase K (PK -resistant aggregates in vitro and in vivo, confirming the PK -resistanterizaggregateshe mutants as prefinrillar αSynuclein vitronts by biophysicandmethods and in invivoebrates [ 22].
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Based on the extent of this overlap and prior characterization of these gradients [ 16] we operationally define the PM pool in these gradients to be in fractions 15-20.
Because prior characterization of this TAI model was in C57BL/6 mice, we first confirmed that the closed skull impact generated appropriate TAI within the corpus callosum of Gli1-CreER T2 mice, which are on an outbred genetic background.
Western blot analysis confirmed that differences in neurotoxicity were not due to different levels of tau expression (Figure S1), consistent with prior characterization of these mutant lines [13], [19], [20].
Prior characterization of this line suggests normal developmental growth and response in adult hearts to pressure overload.
Prior characterization of this isolate demonstrated that it has a PQRETR/GLF HA0 cleavage site (GenBank accession no. DQ826532) typical for LPAI viruses.
Although reagents that would enable direct visualization of the O-fucosylation state of Notch in cells expressing Ofut1 R 245 A do not exist, prior characterization of this mutant isoform indicates that it is completely defective in fucosylation [ 10].
We chose this number based on our prior characterization of these networks where we classified genes as hubs or bottlenecks if their degree or betweenness centrality scores, respectively, were statistical outliers [ 17].
Critical to this effort was the prior characterization of two elements of the network, TL-1 and TL-2.
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