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Distal femoral nails cannot address these injuries due to the low fracture lines with articular comminutions, and the need for free multidirectional lag screws for prior assembly of the fragments that would interfere with insertion of these nails.
Sequence reads can then be assembled in the case of a low-complexity sample [10], or assigned to taxonomic groupings using various binning strategies without prior assembly [5], [7], [11].
To obtain a quantitative picture of the taxonomic composition, sequence datasets were characterized without a prior assembly step [ 56].
The metagenomic reads were utilized without prior assembly software analysis by the COMPAREADS software (Maillet et al. 2012).
In this work, we demonstrate the effectiveness of Illumina NGS paired-end sequence reads for rapid and cost-effective microsatellite development with and without prior assembly of reads.
These results suggest that both the prior assembly of 454 data and the current assembly of PacBio data had unique regions not covered by the other assembly.
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Prior to assembly submission each assembly is gap filled and cleaned of vector and contaminating contigs.
With repeats masked prior to assembly, the speed of assembly was increased with lower error probability.
Moreover, this assembler corrects reads prior to assembly and enables a reduction in the 'trustable coverage threshold' for low coverage regions.
We discovered that filtering reads prior to assembly reduces the runtime and memory required by the assembly at the cost of slightly decreasing the assembly quality.
Consolidation of identical reads into a single representative sequence prior to assembly reduces the computational resource requirements for the assembly.
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