Exact(3)
The aging profile was similar to that found in prior array studies.
Degeneration of pontine tegmentum nuclei antecedent to the death of nigral dopaminergic neurons would generate a prior array of non-motor symptoms, such as rapid eye movement sleep behavior disorder (RBD), a condition that causes the loss of atonia during sleep.
Indeed, prior array comparative genomic hybridization analysis of both cell lines had revealed that the ALMC-2 cell line had lost a copy of the X-chromosome, whereas the KAS-6/1 cell line retained both intact X-chromosomes (unpublished data).
Similar(57)
We confirmed some previous findings while not others, in addition to identifying novel differentially methylated CpGs at these genes of interest, due to the much higher depth of sequencing coverage in BisPCR compared to prior array-based approaches.
Array genotyping necessarily has a data acquisition bias; variants must be identified prior to array design, limiting the array capture to known variation which may be optimally informative for only the populations used for variant discovery.
Prior to array staining, the sera were diluted to 1/100 in Protein Array Blocking Buffer (Schleicher & Schuell Bioscience) containing Escherichia coli lysate at a final concentration of 10% and incubated at room temperature for 1 h with constant mixing.
Feline sera were preabsorbed with E. coli lysate prior to array staining, to remove background reactivity to E. coli proteins in the IVTT reactions.
Prior to array hybridization, TRIM28 (KAP1), SETDB1 and G9a ChIP DNA was amplified using the Sigma GenomePlex WGA2 kit as described in O'Geen et al. [56].
To maximise ability to capture of variability accounted for primarily by biological factors only, it was considered prudent to normalise input RNAs of all samples prior to array probe generation.
Prior to array analysis we scored all samples for hMAM expression.
Prior to array analysis, one round of T7 promotor-based RNA amplification was performed [ 21].
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