Sentence examples for prior adhesion from inspiring English sources

We also considered whether any possible prior adhesion of human monocytes to endothelial cells, as an initial step of migration, could initiate the translocation of HIF-1α into the nucleus.

The platelet suspension was pretreated with either a GPIIb/IIIa-antagonist drug Aggrastat® or monoclonal antibodies 6B4 or 24G10 against GPIb-IX-V prior to adhesion on Fg- and Alb-coated OH- and CH3-functionalized alkanethiol self-assembled monolayer surfaces.

For simulating the influence of a salivary pellicle, we incubated specimens in 48-well plates with 1 ml of artificial saliva for 2 h prior to adhesion testing [2].

Chicken fibroblast (CEC-32) cells [41] were used between passages 6 and 9 and were seeded into 12-well microtitre plates at a density of ∼2×105 cells per well and incubated at 37°C in an atmosphere containing 5% CO2 without antibiotics prior to adhesion assays.

Rolling of cells prior to adhesion was not observed.

Tumour cells and peripheral blood mononuclear cells were fixed in suspension prior to adhesion to the slide.

To obtain an equilibrium surface state, more than 300 contacts were made between a smooth PDMS sample and the glass plate prior to adhesion measurements.

To determine whether adhesion-enhancing CD44 antibodies imposed their effect indirectly via triggering of intracellular signalling cascade, the experiment above was repeated with the antibody preincubation step at 37 or 4 °C prior to adhesion assay.

The following incubation times were used for inhibitors added prior to adhesion of platelets: apyrase (2 U/ml for 1 min), indomethacin (10 μM for 5 min), ODQ (20 μM for 15 min) or myrPKI (500 nM for 15 min).

Then, the samples were only washed with water steam under pressure to remove any possible contaminants present on the surfaces and then stored in distilled water at 37°C for 24 h, prior to adhesion assays and biofilm formation.

For integrin blocking studies, cells were premixed with azide free/low endotoxin (BD Biosciences, San Jose, CA) antibodies (Ha31/8 for α1 and 5H10-27 for α5) or control IgM at 10 μg/ml and incubated for 30 minutes at room temperature prior to adhesion.