Sentence examples for prior addition from inspiring English sources

For in vitro experiments with lovastatin, cells were incubated with 25 μM of the active form of lovastatin (Millipore) for 4 h prior addition of trastuzumab40.

IMD affected cAMP generation in the myocardium and aorta, which were not attenuated by prior addition of either CGRP8 37 or ADM22 52 alone.

Prior addition to the silane solution, the CNTs were submitted to a treatment in an aqueous solution containing cerium nitrate or lanthanum nitrate.

Electrical field stimulation (EFS, 40 V, 0.5 ms, 2 Hz) produced contraction (79.8 ± 7.1 g ten- sion · g−1tissue) in the bladder rings that can be greatly reduced by prior addition of ATP or AP4A.

However, with the prior addition of magnesium (5 and 10 mM) for 2 h, the elevated nuclear translocation of NF-κB was inhibited in LPS- and IFN-γ-stimulated cells, with decreases in the nuclear:cytoplasmic ratio to 0.88 and 0.90, respectively (Fig. 6A,B).

The pH of all the media was adjusted to 5.7 ± 0.1, prior addition of agar and before autoclaving for 15 min under 1.05 kg/cm at 121°C.

Addition of ATP prior to addition of AP4A or vice versa desensitized bladder tissue to the second agonist with great reduction in the contraction produced.

Prior to addition of the probes, NucBlue Live ReadyProbes Reagent was used for live cell detection.

The pH was adjusted to 6.5 with H3PO4 or to 7.4 with NaOH prior to addition to cells.

Incoming immune cells were pre-treated with vehicle or B49Mod1 for 2 hours prior to addition to fibronectin-coated plates.

HEK293FT cells were co-transfected with inducer and reporter proteins for 6 h prior to addition of the small molecules.