Sentence examples for printed spot from inspiring English sources

Expression data were omitted in the regions where no signal was present or if the signal was just above local background or derived from <40% of the area of the printed spot.

Early issues with variation in printed spot morphology were resolved by pre-rinsing the filters: apparently this removes material acting as a surfactant and effects the spot morphology and/or drying characteristics.

A few spots from these arrays were excluded from analysis when both fluorescence signals were within two standard deviations of the distribution of the intensities of the background pixels of that spot, or if printed spot morphology was poor.

This difference can vary from a subtle decrease in intensity under the printed spot to a much more predominant intensity variation that seriously affects the accuracy of the data.

Deviations in printed spot positions are detected by imaging each printed slide using a digital camera.

Finally, although we analyzed images to gather population data across the cells in a printed spot, it is clear that single-cell data could be gleaned using more sophisticated image processing techniques [26], [27].

Immediately after printing, slides were imaged with transmitted light to analyze print quality; printed spots were discrete and typically contained 20 50 cells.

The Bovine Innate Immune Microarray contains 16,128 individually printed spots.

Resulting images were analyzed using GenePix 3.0 or 4.0 (Axon) to locate and quantify the printed spots.

This hybridization visualized the size and shape of all printed spots, which allowed us to ensure uniformity in the amount of DNA spotted by each pin and to check the integrity of each spot.

Printing spots were uniform as assessed by SYTO-6 staining.