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AG: antigen; CRC: colorectal carcinoma; Ca: carcinoma; 2D: two dimensional; DIGE: 2D fluorescence difference gel electrophoresis (GE healthcare system); ESI: electrospray ionisation; KS: control sera; MW: molecular weight; MALDI: matrix-assisted laser-desorption ionisation; PMF: protein mass finger print; Seq cov: sequence coverage; SERPA: serological proteome analysis.
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Unlike microarrays, which are limited to the genes printed on the chips, mRNA-seq represents an open platform that is capable of detecting novel transcripts, provided that they are expressed at levels compatible with the sequencing depth.
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