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The principles of fluorescence microscopy.
This chapter describes the basic principles of fluorescence resonance energy transfer (FRET), focusing on both steady-state and time-resolved methodologies.
This chapter outlines the basic principles of fluorescence resonance energy transfer (FRET), focusing on both steady-state and time-resolved methodologies.
Such sensors can be operated on the principles of fluorescence, electro-cheumuluminescence, and electrochemistry with many advantageous features (e.g., robust biointerfacing through various conjugation chemistries, facile sensor assembly, high stability with regard to temperature/pH, and high affinity to target).
Documentation of the Jurassic specimens followed the principles of fluorescence composite imaging and macro-fluorescence imaging (see [ 26],[ 27]).
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Two general principles of fluorescence-based protein detection have been developed: reactive dyes that couple with protein amines to form fluorescent, covalent adducts, and dyes that interact non-covalently with hydrophobic regions of proteins or at a protein-detergent interface.
The first category uses the principle of fluorescence resonance energy transfer (FRET) between two variants of the green fluorescent protein (GFP), covalently linked with Ca2+ binding proteins like calmodulin [29], [30].
In this review, several approaches are introduced including a recently described fluorescent antibody-based biosensor Quenchbody, which works on the principle of fluorescence quenching of attached dye and its antigen-dependent release.
In this review, following a brief outline of the principle of fluorescence imaging, we recount some recent achievements in the field of small-molecular fluorescent probes.
We report the construction of a cell-based fluorescent reporter for anthrax lethal factor (LF) protease activity using the principle of fluorescence resonance energy transfer (FRET).
B Principle of fluorescence resonance energy transfer (FRET).
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