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Fang et al. now apply these principles in zebrafish to develop an efficient system for testing new atherosclerosis therapies.
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As a first step, we examined the proteasomal inhibitor MG132 as a proof of principle in the zebrafish system comparing wild-type with dystrophic sapje larvae.
Here we provide further proof-of-principle that chemical screens in zebrafish can uncover novel anti-angiogenic drugs for the eye.
As expected, genes with proximal binding in zebrafish show enriched expression at the principle sites of Nodal activity in early zebrafish embryogenesis, such as the margin and shield.
A proof of principle screen identified a number of putative mutants, making funduscopy based screens in zebrafish feasible.
In principle, the use of our red-to-green reporter line should allow genetic fate mapping in zebrafish in the same manner as in mouse.
This analysis provides an important proof of principle for genomic modifications in this important model for vertebrate biomedical research; we expect that precise genome editing in zebrafish is possible at other loci with similar efficiencies.
When these cells formed a tumour in zebrafish, they saw the green cells physically helping the slower-spreading red cells break away from the tumour and spread.
Additionally, it elicited hypoactivity in zebrafish larvae.
In zebrafish, all antidepressants were anticonvulsant in the pentylenetetrazole model.
Moreover, according to the results of zebrafish model system, glabridin shows no effects on melanin synthesis in zebrafish but presents toxicity to zebrafish embryo.
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