Sentence examples for principal genetic groups from inspiring English sources

Isolates were assigned to Principal Genetic Groups (PGG) after PCR and subsequent SNP analysis of katG463 and gyrA95 using primers as described in Sreevatsan et al [21] (Table 1).

Isolates were assigned to principal genetic groups according to the polymorphisms in the katG and gyrA genes (11).

The T clade corresponds to Principal Genetic Groups (PGG) 2 and 3, and remains defined by default (no specific signature sequences).

Sreevatsan et al. divided MTBC strains into 3 principal genetic groups (PGG1 PGG3) based on SNPs in codon 463 of katG and codon 95 of gyrA (2 ).

Sampson, unpublished results) and a recent study by Talarico et al. [ 31] found sequence variation for PE_PGRS33 (Rv1818c) in 68% of clinical isolates spanning all three M. tuberculosis principal genetic groups [ 32].

Genotyping with the 25 extra spacers revealed that the "T1-ancestor" or "Manu" isolates all harbored the same signature (absence of spacers 54-61) as do isolates from Principal Genetic Groups 2 and 3 modern isolates.

The prevalence of each principal genetic group (GG) was 30% (GG1), 52% (GG2), and 18% (GG3).

Phylogenetically the SMI-049 isolates were of the modern principal genetic group 2 (PGG2) defined by katG463 and gyrA95 SNPs (katG463 CGG (Arg) and gyrA95 ACC (Thr)) [45].

Principal genetic group 1 (PGG1) strains, such as the W-Beijing, CAS, EAI and M. bovis families of strains, appear to be only very distantly related to the strains of PGG2 and PGG3, providing a strong argument for the use of ecotype categorization for MTC members rather than the traditional subspecies classification.

*RFLP, restriction fragment length polymorphism; PGG, principal genetic grouping; WT, wild type (identical to strain H37Rv); ND, not determined.

The SNPs in codon KatG463 (CGG) and codon gyrA95 (AGC) revealed that the MTZ strain belonged to the principal genetic group 3.