Gene expression profiling of isolated aortic EC identified cell cycle and cell migration as the principal biological processes affected in the double mutant EC.
Identifying proportions of essential gene-containing orthologous groups in GO functional categories revealed significant enrichment of essential genes among principal biological processes in mouse and fly (fig. 3, supplementary table 5, Supplementary Material online).
To identify principal biological processes associated with age-related transcriptional changes, we first evaluated top-ranked genes via Pathway Explorer (Mlecnik et al., 2005), a web-accessible tool that allows life scientists to infer the biological meaning behind large gene lists.
Based on the Gene Ontology ID, unique sequences were categorised into three principal categories: biological processes, cellular localizations and molecular functions.
Based on the Gene Ontology ID, unique sequences were categorized into three principal categories: biological processes, cellular localizations, and molecular functions.
On the basis of GO categories, we assigned oak ESTs containing SSR motifs (Ueno et al. [ 33]) to three principal groups: biological processes, cellular compounds and molecular functions.
In our analysis, we grouped all contigs according to the highest sub-category under the principal GO categories: Biological Process, Cellular Component, and Molecular Function.
Therefore, the "level" presented in this study corresponds to the depth of hierarchical categories in each principal GO category, with the topmost Level 1 representing the most general classification (principal GO categories) of biological process, molecular function, and cellular component.
Between parentheses: the principal subcategory of the biological process "metabolism" associated to the annotation.
The Gene Ontology annotation of transcripts helped classify functional descriptions into three principal ontologies: molecular function, biological process and cellular component.
