2. Influence of media composition on primordial follicle survival Ovarian pieces from an Amur leopard were used to analyze the impact of protein source on follicles survival during prolonged culture.
The results reflect a mix of curiosity, empathy, humor, and, often, an ineffable longing for the primordial purity, survival skills, or simple integrity of purpose that Foster describes.
Given the recent evidence that S1P may play a positive role in angiogenesis in non-reproductive cell lines and tissue [13], [15], [23], [31], [32], [33], we hypothesized that S1P and its agonists could improve neoangiogenesis, and as a result, primordial follicle survival during human ovarian cortical tissue transplantation.
The results of total follicle distribution during culture and the relative primordial follicle survival in different culture experiments in ovarian cortex pieces were analyzed by one-way ANOVA.
They reported that no significant difference in the primordial follicles survival rate and the apoptosis rates was found between the two cryopreservation groups.
Further experimentation is therefore required to determine whether more aggressive anti-thrombotic regimes, involving longer periods of post-operative treatment and/or higher doses, would have a further beneficial effect on primordial follicle survival following WOCP&TP.
However, the rate of restoration of ovarian function (25%) and the relatively low rate of primordial follicle survival in cycling animals (29%) were still far below the levels needed for this technique to be adopted as a clinical intervention.
In contrast, the duration of CPA perfusion was highly significant with a 60 min perfusion resulting in ovaries of normal appearance and function with high rates of primordial follicle survival (70%) and an abundant blood supply, whereas ovaries perfused for 10 min had either resorbed completely and were vestigial (7/14) or were markedly smaller (P < 0.01).
Kehler, J. et al. Oct4 is required for primordial germ cell survival.
