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The suggested focus counting and calculation of follicle number per piece and the primordial proportion from the overall follicular population might be used to compare treatment protocols, although alternative approaches should also be considered.
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The distribution reflects the probability of the cells being in each attractor, and may be interpreted as the proportion of primordial cells fixed to each GRN configuration.
Quantification of follicle numbers showed that the proportion of primordial follicles in rapamycin-treated Oo Tsc1−/− ovaries was elevated to 73%, which was similar to the proportion in Oo Tsc1+/+ ovaries (74%) (Fig. 6F).
Results: Proportions of primordial follicles were significantly lower in grafts than in the tissue before transplantation in gonadotrophin-stimulated mice (37% versus 79%), but not in non-stimulated mice (51% versus 74%).
The combined treatment of CTGF and TGFβ-1 was found to increase the proportion of assembled primordial follicles and decrease the proportion of unassembled follicles (Figure 4B and 4C).
On examination, the proportion of type 1a follicles (activating primordial follicles) was significantly lower in animals from the CT groups compared with CC and TT groups (P < 0.05).
The quantity of elements heavier than helium is not known precisely, but the proportions are assumed to match the primordial abundances from the formation of the Solar System.
In Oo Tsc1−/−; Pdk1−/− ovaries of the same age, however, clusters of primordial follicles were seen (Fig. 8D, arrows), the proportion of which was elevated to 52% (Fig. 8E).
In Oo Tsc1−/− ovaries, all primordial follicles were activated by PD23 (Fig. 8B, arrows), and the proportion of primordial follicles (compared with the total number of follicles) was 0% (Fig. 8E).
Unfortunately, calculations carried out under the inhomogeneous hypothesis seem to indicate that conditions leading to the correct proportions of deuterium and helium-4 produce too much primordial lithium-7 to be compatible with measurements of the atmospheric compositions of the oldest stars.
In primordial follicles, labeling of some follicular epithelial cells was also observed, while higher proportions of granulosa cells were labeled in primary and secondary follicles.
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